Organization: Astrazeneca Ease of use 5 out of 5 After sales service 4 out of 5 Value for money 5 out of 5 Great results Rating: 4. Organization: Hospital for Sick Children Ease of use 5 out of 5 After sales service 5 out of 5 Value for money 5 out of 5 Great instrument and assays, reliable and easy to use!
Organization: Smithsonian Institution Ease of use 5 out of 5 After sales service 5 out of 5 Value for money 5 out of 5 Great results!!!! I use it for all my quantifications!
The integrated design of the Qubit Fluorometer and assays results in quantitation that is far more sensitive than UV absorbance, making this system essential for quantitation of precious samples samples that are rare, difficult to purify, or expensive to either obtain or prepare or samples for "delicate" applications samples for downstream assays that are extremely sensitive to sample conditions.
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Write your own review. Reviews Description. Great user interface and easy to use and interpret results. Good user interface. It fits you. Amr Abid: Qubit 2. Voice: Much time, effort, and research money is invested to obtain precious nucleic acid and protein samples.
We developed Qubit 2. Amr Abid: The device provides trust and confidence ensuring downstream applications don't suffer from inaccurate quantification. Qubit 2. Basic Qubit protocol is simple and shouldn't take more than two minutes. The first step is to prepare the working solution. Use microliters of buffer for each tube. Use 1 microliter of dye for each tube. Because we have five tubes in our DNA quantification assays, we will need 5 microliters of dye and 1 mL of the buffer.
Mix the dye and the buffer in 1. Now we're ready to add the standards and the DNA sample. Use thin-wall PCR tubes. For DNA quantification assay, we need two standards.
You can also trigger the stand-by mode by touching the Power button located on the top left of the touch screen. Press the Version button located on the top portion of the touch screen. Running New Ensure that you are using the Standard 1 appropriate for the Standards for assay you are performing. The reading takes approximately Calibration, 3 seconds. The most recent sample is signified by a large black circle.
The Reading measurement takes approximately 3 seconds. Samples, continued Upon the completion of the measurement, the result is displayed on the screen. The number displayed is the concentration of the nucleic acid or protein in the assay tube. Concentration graph and the screen only displays the latest measurement. To calculate the concentration of your original sample, press Dilution Calculate Stock Conc.
A pop-up window Calculator, showing the current unit selection as indicated by an adjacent continued red dash opens. Select the unit for your original sample concentration by touching the desired unit in the unit selection pop-up window. CSV file and tagged with a time and date stamp. CSV comma separated value file for sample comparisons. Each measurement data point in the. CSV file is numbered and exhibits a time and date stamp. Page 26 Data Handling, Continued Select the data file to rename by touching the corresponding Renaming Data line of the spreadsheet on the Data Screen.
The selected line Files will be highlighted. If no lines are selected, you are prompted to select a line or multiple lines to rename.
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